collagenase type ii (Thermo Fisher)

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Thermo Fisher collagenase type ii
Collagenase Type Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/type+ii+collagenase/Collagenase/pmc12809746-140-38-41
Average 97 stars, based on 1 article reviews

collagenase type ii - by Bioz Stars, 2026-09

97/100 stars

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Cell Differentiation:

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation.
Article Snippet: The cells were then analyzed using a BD FACSCelesta cytometer. .. For analysis of Th17 cell differentiation of CD4+ T cells in joint tissues, the mouse ankle joints were dissected and digested with 0.5% type II collagenase (GIBCO, # 17101-015). .. The discrete cells were collected, stimulated with Cell Stimulation and Protein Transport Inhibitor Cocktail, and then stained with live/dead violet dye (BioLegend, #423113), anti-CD4-PE (BioLegend, #100407) and IL17A-APC (BioLegend, #506915).

Cell Culture:

Article Title: VAP1 promotes cardiac fibrosis by enabling PDGFR signaling in myofibroblasts.
Article Snippet: .. Briefly, the ventricles were dissected from 8-week-old C57B/6j mice and minced into small pieces with a microscopic scissor and then digested with type II collagenase (17101015, Thermo Fisher) for 60min in a cell culture incubator. .. The digested cells were passed through a 100 μm cell strainer (352360, Corning, Charlotte, NC, USA) and pelleted by centrifugation.

Suspension:

Article Title: Development of Intervertebral Disc Organoids through Directed Differentiation of Mesenchymal Stem Cells and Hierarchical 3D Printing
Article Snippet: Human NP, AF and CEP samples classified as grade I were obtained from volunteers who were diagnosed with idiopathic scoliosis, as previous described ( Table S1 )., NP, AF and CEP samples obtained via were immersed in phosphate-buffered saline (PBS; Biosharp, China) and minced into 1–3 mm 3 fragments. .. The fragments were washed three times with PBS to remove residual debris, followed by digestion with 0.4% Type II collagenase (Invitrogen, USA) or Type I collagenase (Invitrogen, USA) at 37 °C for 4–6 h. The resulting cell suspension was centrifuged at 800 rpm for 5 min, washed three times with PBS, and resuspended in growth medium (DMEM/F12, 1:1; Gibco, USA) supplemented with 15% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin-streptomycin (Gibco, USA). ..

Article Title: A ROS and pH dual - responsive hydrogel-nanoparticle system restores intervertebral disc and alleviates related pain by modulating senescence and panoptosis of nucleus pulposus cells
Article Snippet: Briefly, freshly obtained healthy NP tissue was placed in PBS (phosphate-buffered saline, Biosharp, China) and cut into 1–3 mm 3 pieces. .. Tissue pieces were washed three times with PBS to remove surface residues, then digested with 0.4% type II collagenase (Invitrogen, USA) at 37 °C for 4–6 h. The resulting cell suspension was centrifuged at 800 rpm for 5 min and washed three times with PBS before being resuspended in culture medium as described above. ..

Article Title: Fmoc-DDT@Fos hydrogel mitigates temporomandibular joint osteoarthritis through regulating macrophage reprogramming and ferroptosis
Article Snippet: .. Condylar cartilage was obtained from Sprague-Dawley (SD) rats (Shanghai SIPPR-Bk Lab Animal Co., Ltd., China) under sterile conditions, rinsed with PBS, cut into pieces, and digested with 0.2% type II collagenase (17101015, Thermo Fisher, USA) and 0.25% trypsin (25200072, Thermo Fisher) at 37 °C for 3 h. The resulting cell suspension was filtered through a stainless steel mesh and centrifuged for 5 min. .. The cells were subsequently transferred to Dulbecco's Modified Eagle Medium (DMEM, 11965092, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS, 10099, Gibco, USA) and 100 U/mL penicillin-streptomycin solution (10378016, Thermo Fisher) and incubated with 5% CO 2 at 37 °C.

Cell Isolation:

Article Title: Soy isoflavones inhibits MMPs expression in keratoconus keratocytes through NF-κB pathway.
Article Snippet: Keratoconus (KC) is a progressive ocular disorder characterized by central corneal thinning and anterior protrusion.. The available clinical treatment options for KC remain limited, and there is currently a paucity of effective pharmacological intervention strategies.. To address this therapeutic challenge, we identified through small-molecule screening that soy isoflavones (Si) significantly inhibit the release of corneal matrix metalloproteinases (MMPs) and enhance the biomechanical stiffness of the corneal stroma.

Saline:

Centrifugation:

Incubation:

Article Title: Piezo2 Mediates a Vicious Cycle of "Mechanical Homeostasis Imbalance-Inflammation" in Sensory Nerves and the Cartilage Endplate.
Article Snippet: .. The tissues were digested in 2 mg/mL 0.1% type II collagenase (Gibco, 17101015) at 37◦C for 4 h. The digested cartilage tissue was then incubated in DMEM supplemented with 10% fetal bovine serum (Hyclone) and antibiotics (1% penicillin/streptomycin), and the mixture was maintained at 5% CO2 and 37◦C. ..

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